Spatiotemporal regulation of the cellular RNAs is crucial for proper protein production and cellular function. However, the intricate subcellular dynamics and regulatory mechanisms of RNA life cycle remain largely obscured due to the limitations of existing analytical methods. Thus I aim to create a panoramic map of the RNA life cycle at genome-wide scale through developing and applying an in situ RNA sequencing toolbox to trace RNA synthesis, processing, export, transport, translation, and decay. This study will advance our understanding of RNA-centered gene regulation mechanisms that shape cell structure, state, and fate, and further, dissect the role of RNA metabolism in tissue function and dysfunction.